Table 2.
Lower limit of detection (LLOD), linearity, precision for two standard concentrations (n = 5) and method reproducibility for 5 samples of Populus leaf and wood tissue
| Compound | LLOD (pmol) | Linearity range pg; R2 | 125 pg (%RSD) | 2500 pg (%RSD) | Populus leaf (%RSD) | Populus wood (%RSD) |
|---|---|---|---|---|---|---|
| 3-Phosphoglyceric acid | 0.067 | 50–10,000; 0.98 | 6.9 | 4.1 | 7.6 | 16.4 |
| 2-Phosphoglyceric acid | 0.007 | 50–10,000; 0.99 | 8.7 | 8.1 | 14.8 | 22.7 |
| 3-Phosphoglyceraldehyde | 0.011 | 50–10,000; 0.99 | 5.7 | 3.5 | 14.7 | 7.8 |
| Dihydroxyacetone phosphate | 0.022 | 50–20,000; 0.99 | 4.9 | 3.6 | 7.7 | 6.1 |
| Erythrose-4-P | 0.045 | 50–5000; 0.99 | 6.4 | 4.2 | 10.7 | 29.8 |
| Ribulose-5-PP | 0.091 | 250–20,000; 0.99 | na | 4.3 | 12.6 | 8.1 |
| Fructose-1,6-PP | 0.25 | 500–20,000; 0.99 | na | 5.6 | 14.3 | 7.7 |
| Xylulose-5-P | 0.054 | 50–5000; 0.99 | 6.6 | 7.4 | 17.9 | 20.2 |
| Ribulose-5-P | 0.054 | 50–5000; 0.99 | 4.2 | 2.1 | 5.9 | 8.9 |
| Galactose-1-P | 0.029 | 50–5000; 0.99 | 7.4 | 2.5 | 11.2 | 4.8 |
| Ribose-5-P | 0.026 | 50–5000; 0.99 | 4.6 | 3.1 | 16.7 | 7.8 |
| Glucose-1-P | 0.024 | 50–5000; 0.99 | 1.6 | 2.2 | 18.9 | 11.5 |
| Fructose-6-P | 0.012 | 50–20,000; 0.99 | 1.5 | 1.8 | 7.8 | 3.5 |
| Glucose-6-P | 0.005 | 50–20,000; 0.99 | 3.4 | 2.6 | 5.7 | 4.1 |
| Seduheptulose-7-P | 0.008 | 125–20,000; 0.99 | 2.9 | 1.7 | 14.5 | 9.7 |
| Trehalose-6-P | 0.009 | 50–20,000; 0.99 | 5.7 | 1.9 | 16.2 | 11.0 |
| Sucrose-6-P | 0.011 | 50–5000; 0.99 | 6.6 | 2.9 | 21.1 | 17.8 |
| UDP-glucose | 0.074 | 125–10,000; 0.97 | 6.5 | 4.7 | 8.7 | 8.1 |
%RSD relative standard deviation in percentage