Figure 1.

Overview of capped small RNA-seq. (A) Schematic of short initiated RNAs that are captured by csRNA-seq and (B) a graphical depiction of the method starting from total RNA. (C) Transcription start site (TSS) clusters are determined through the enrichment of small capped RNAs over the total small RNA input using HOMER. The schematic shows the typical distribution of csRNA-seq and input at various genomic features. (D) Integration of genome annotations or total RNA-seq enables the assignment of TSSs to stable and unstable transcripts.