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. 2019 Oct 31;10:1226. doi: 10.3389/fphar.2019.01226

Figure 4.

Figure 4

Dihydroisotanshinone I (DT) induces apoptosis in breast cancer cells. MCF-7 cells or MDA-MB-231 cells were treated without or with indicated compounds for 24–48h. Cell apoptosis was detected by flow cytometry with annexin-V-FITC/PI dual staining or mitoscreen JC-1 staining. (A) For annexin-V-FITC/PI dual staining, the representative histograms of flow cytometric analysis using double staining with annexin-V-FITC (FITC-A) and PI (PI-A). (B) For mitoscreen JC-1 staining, dot Plots revealing depolarization of mitochondria in treated indicated breast cancer cells. The percentage of events in the upper gate (P2) and lower gate (P3) represent population of treated indicated breast cancer cells having normal and depolarized mitochondria respectively. (C, D) Total cell extracts of MDA-MB-231 cells (C) or MCF-7 cells (D) were harvested from cells treated with DMSO or indicated concentrations of DT for indicated hours. The protein was immunoblotted with polyclonal antibodies specific for PARP. β-actin was used as an internal loading control.