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. 2019 Oct 31;10:1226. doi: 10.3389/fphar.2019.01226

Figure 5.

Figure 5

DT induced ferroptosis in breast cancer cells in vitro. (A, F) For MDA assay, MCF-7 cells (A) or MDA-MB-231 cells (F) were treated with DMSO or indicated drugs for 24 hours. Total cell extracts of indicate breast cancer cells was collected and analyzed by MDA assay kit. (B) For GPX activity, MCF-7 cells were treated with DMSO or indicated drugs for 24 hours. Total cell extract was collected and analyzed by GPX activity assay kit. (C, G) Total cell extracts of MCF-7 cells (C) or MDA-MB-231 cells (G) were harvested from cells treated with DMSO or indicated concentrations of DT for 24 hours. The protein was immunoblotted with polyclonal antibodies specific for GPX4. β-actin was used as an internal loading control. (D, E, H) For GSH and GSSG level, indicated breast cancer cells were treated with DMSO or indicated drugs for 24 hours. Total cell extract was collected and analyzed by GSH and GSSG assay kit. (Error bars=mean±S.E.M. Asterisks (*) mark samples significantly different from DMSO group with p < 0.05).