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. 2019 Sep 16;181(3):976–992. doi: 10.1104/pp.19.00911

Figure 1.

Figure 1.

Molecular characterization of NTRC-RNAi lines 2, 26, and 33 compared to the wild type (WT) in 35 DAF tomato fruits. A, The RNAi construct targeting the NTRC gene. B, Genotyping by PCR analysis for detection of the nptII gene in NTRC-RNAi lines. C, Detection of the NTRC transcript level using RT-qPCR. D, Detection of NTRC proteins by immunoblot analysis. E, Quantification of NTRC protein abundance using Image-J software. The level of clathrin adaptor complexes (CAC) serves as a reference gene for genotyping and RT-qPCR. The protein level of ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (RbcL) served as a loading control for immunoblotting. Results are the mean ± se from six biological replicates. Statistical analysis used ANOVA with Dunnett’s test (*P < 0.05, compared to the wild type).