Table 1. Photosystem absorption cross section and Chl a content per P700 in alb3b mutants compared with wild-type cells.
Photosystem absorption cross section was measured as rate of 533-nm (Fx) or 670-nm (Chl a) photons absorbed by the functional thylakoid membranes. The actinic light intensity was adjusted to I670 = 2.1 µmol photons m−2 s−1 and I533 = 12 µmol photons m−2 s−1. Rates of light absorption and utilization are given in photons per second ± sd. P700 quantification was measured from the light-induced ΔA700 with 670-nm (Chl a) actinic illumination.
| Photosystem | Parameter | Wild Type | alb3b-14 | alb3b-16 | alb3b-19 | Average alb3b | alb3b/Wild Type |
|---|---|---|---|---|---|---|---|
| PSI | (Fx) 533 nm | 2.61 ± 0.40 | 1.10 ± 0.08 | 1.17 ± 0.10 | 1.09 ± 0.00 | 1.10 ± 0.06 | 42% |
| (Chl) 670 nm | 1.93 ± 0.11 | 1.43 ± 0.05 | 1.39 ± 0.15 | 1.39 ± 0.14 | 1.40 ± 0.01 | 72.5% | |
| PSII | (Fx) 533 nm | 32.30 ± 0.7 | 13.92 ± 1.96 | 8.10 ± 1.16 | 8.58 ± 0.93 | 10.17 ± 3.24 | 35% |
| (Chl) 670 nm | 12.62 ± 2.69 | 7.21 ± 0.00 | 7.08 ± 0.90 | 6.71 ± 1.36 | 7.00 ± 0.26 | 55% | |
| Chl a/P700 | 663% ± 9%:1 | 466% ± 11% | 414% ± 9% | 394% ± 11% | 425:1 | 64% | |