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. 2019 Sep 6;181(3):1239–1256. doi: 10.1104/pp.19.00458

Figure 2.

Figure 2.

The wer-4 mutant affects the expression of WER target genes. A, Relative amounts of ETC1, TRY, GL2, and CPC transcripts in seedling root tips of the wild type (WT), wer-4, and wer-1 determined with RT-qPCR. Error bars represent sd from three replicates. Statistical significance was determined with one-way ANOVA: ***, P < 0.001; ns, not significant. B, ETC1::GUS transcriptional reporter expression in seedling root tips of the wild type and wer-4. Bar = 50 μm. C, Expression of GL2::GUS and GL2::GFP transcriptional reporters in wild-type and wer-4 seedling root tips. Stars indicate H-position cell files. In the fluorescence image, the red color represents propidium iodide and the green color represents GFP. Bars = 50 μm. D, Expression of CPC::GUS transcriptional reporter in wild-type and wer-4 seedling root tips. Stars indicate H-position cell files. Bar = 50 μm. E, Histograms of GL2::GFP signal levels in N- and H-position epidermal cells of wild-type and wer-4 seedling root tips (n = 170). Ten cells in H and N positions were analyzed per root, and 17 roots were analyzed for each genotype. F, Histograms of CPC::GUS signal levels in N- and H-position epidermal cells of wild-type and wer-4 seedlings root tips (n = 240). Ten cells in H and N positions were analyzed per root, and 24 roots were used for each genotype.