Figure 3.

Knocking out GFR1 in Nipponbare decreases the grain-filling rate and grain weight. A, T-DNA expression construct for Cas9 and gRNA for the GFR1 gene in rice. The Cas9 nuclease is under the control of the doubled 35S promoter, and transcription was terminated by the nos terminator. The rice U6 promoter (OsU6) was used to drive the expression of the single guide RNAs (sgRNAs). LB, Left border; RB, right border. B, Sequence comparisons of the targeting site of the KO plants generated by CRISPR-Cas9 technology. The two sgRNA target sequences are underlined in blue and green. WT, Wild type. C, Comparison of the brown rice weight and grain-filling rate changes of Nipponbare and KO plants at five stages. The data are means ± sd (n = 3). D, Morphological changes in the brown rice grain of Nipponbare and KO plants at the five grain-filling stages. Bar = 1 cm.