Figure 4.
Analysis of the expression of GFR1. A, Relative expression levels of GFR1 are shown in the tissues of C-bao and NIL-GFR1Ludao, including grains and flag leaves at five grain-filling stages, and in stems, roots, stamens, and stigmas. The 18S rRNA gene was used as an internal control. The data are means ± sd (n = 3). Student’s t test was used for statistical analysis (*, P ≤ 0.05 and **, P ≤ 0.01). B to J, Promoter activity analysis of GFR1 in NIL-GFR1Ludao. B, Root. Bar = 1 cm. C, Center of flag leaf. Bar = 1 cm. D, Stem. Bar = 1 cm. E, Transverse section of a stem. Bar = 1 cm. F, Young panicle. Bar = 1 cm. G, Spikelet. Bar = 5 mm. H, Stamen. Bar = 5 mm. I, Pistil. Bar = 1 mm. J, Brown rice grains at different DAF. Bar = 5 mm.