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. 2019 Sep 13;181(3):1207–1222. doi: 10.1104/pp.19.00413

Figure 5.

Figure 5.

Characterization of the GFR1 protein in rice. A, Phylogenetic tree of GFR1 in Arabidopsis, rice, and other monocotyledonous species. The phylogenetic tree was constructed using MEGA 5.0. Bootstrap values are shown as percentages at the nodes. The scale bar at the bottom represents the genetic distance. B to E, Subcellular localization of the GFR1-GFP fusion protein. Membrane localization of the GFR1-GFP fusion protein (B), the location of the chloroplast (C), a merged image of the bright field and the chloroplast (D), and a merged image (E) are shown. Bars = 50 μm. F, Y2H assay showing that GFR1 interacts with proteins of the cDNA library. DDO, Control medium (SD/-Trp-Leu); QDO, selective medium (SD/-Ade/-His/-Leu/-Trp/Aba). G, Transactivation activity assays of GFR1. H, Y2H assay showing that GFR1 of C-bao and Ludao interacts with OsRbcS. I, Y2H assay showing that GFR1 of C-bao and Ludao interacts with OsRbcL. AD, Activation domain; BD, binding domain; SD, synthetic dropout. J, Glutathione S-transferase (GST) pull-down assay of GFR1 and OsRbcS. GFR1 expressed as part of a GST fusion protein was pulled down by His-OsRbcS.