Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 23;181(3):1096–1113. doi: 10.1104/pp.19.00919

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Pulldown analysis of the SNAREs and SEC11 with the KC1^67–91 cytosolic domain. Tagged proteins were expressed and purified from Escherichia coli using His-affinity and size-exclusion chromatography before incubation in vitro overnight at 4°C. Shown is one of three independent pulldown experiments with Coomassie-stained SDS-PAGE analysis following separation with Strep-Tactin resin-immobilized KC1^67–91-Flag₆-StrepII. Lane sets are the SDS-PAGE results of (left to right) the pulldown, the resin control, and the loading. Incubations were carried out individually with SYP121^ΔC-Flag-6His, VAMP721^ΔC-Flag-6His, Flag₃-SNAP33-6His, and SEC11-Flag₆-6His. The unrelated iLOV-6His protein (Christie et al., 1998) was included as a control. Note the lower-molecular weight fractions that are common for purified SEC11 (Karnik et al., 2013b).