Figure 7.
SEC11 competes differentially with KC167–91 for cognate SNARE binding. Pulldown assays with KC167–91 alone and with 0.3-, 1-, and 3-fold molar excess of SEC11 together with each of the cognate SNAREs, as indicated (top). Binding was tested with the SNAREs singly (A–C), in binary (d–F), and in tertiary (G) combinations, as indicated. Incubations were carried out overnight at 4°C before separation. Shown are Coomassie-stained SDS-PAGE gels of the bound proteins from one of three independent experiments in each case. Similar results were obtained with the KAT11–63 peptide (Supplemental Fig. S7), and the results of all experiments are summarized in Figure 8.