SlPIF4 silencing affects tomato fruit quality. A to C, Carotenoid (A), tocopherol (B), and °BRIX (C) in ripe fruits (12 d post breaker stage [BR]). D, Ripening time from anthesis to BR stage. E, mRNA abundance relative to wild-type (WT) MG of differentially expressed genes involved in carotenogenesis. Values represent means ± se of at least three biological replicates, each composed of at least four fruits (A, B, and E), 10 individual fruits (C), or 90 individual fruits (D). Significant differences relative to the wild-type control are denoted by asterisks (two-tailed t test; *P < 0.05; **P < 0.01; ***P < 0.001). BR1-12, 1-12 days after BR stage; RIN, RIPENING INHIBITOR; PSY1, PHYTOENE SYNTHASE; GGPPS2, GERANYL GERANYL DIPHOSPHATE SYNTHASE; L6, 35S::SlPIF4-RNAi L6; L17, 35S::SlPIF4-RNAi L17; L20, 35S::SlPIF4-RNAi L20.