IQM1 positively regulates plant defense against B. cinerea and the synthesis of JA. A, Photographs of rosette leaves cut from 4-week-old wild-type (Columbia-0 [Col-0]), iqm1-1 mutant, and pIQM1::IQM1/iqm1-1 complement plants at 3 d after infection with B. cinerea spores. B, Relative lesion sizes measured by ImageJ software at 3 d after inoculation with B. cinerea. The experiments were repeated at least three times, and similar results were obtained. The data are means ± sd of 10 infected leaves. C, Photographs of infected leaves with Trypan Blue staining at 3 d after inoculation with B. cinerea. D, JA levels of 4-week-old wild-type (Col-0), iqm1-1 mutant, and pIQM1::IQM1/iqm1-1 complement plants at 3 d after infection with B. cinerea. The results were obtained from at least 30 independent plants for each genotype by mixed grinding. The data are means ± sd of three replicates. Asterisks in B and D represent significant differences compared with Col-0 (Student’s t test, **, P < 0.01).