Figure 3.

Temporal accumulation of tocopherols and SA upon Psm and Psm avrRpm1 inoculation. Endogenous levels of α-tocopherol (A), γ-tocopherol (B), δ-tocopherol (C), β-tocopherol (D), and SA (E) are shown in treated (local) leaves of Arabidopsis Col-0 plants at 10, 24, and 48 h following MgCl₂ treatment, Psm inoculation, or Psm avrRpm1 inoculation (OD₆₀₀ = 0.005 each) and in distant (systemic) leaves at 48 h post treatment. Values are given in µg g⁻¹ fresh weight (FW) and represent means ± sd of at least three biological replicates. Each replicate sample consisted of tissue from different plants. Six leaves from two plants were pooled for one replicate. Asterisks denote statistically significant differences between control (MgCl₂) values and values of Psm- or Psm avrRpm1-treated leaf samples: ***, P < 0.001; **, P < 0.01; and *, P < 0.05 (two-tailed Student’s t test).