Figure 7.

P. syringae inoculation induces the accumulation of the tocopherol precursors (D)MPBQ, which are absent in vte2 and strongly overaccumulate in vte1. A, Relative amounts of MPBQ (top) and DMPBQ (bottom) in local leaves of Arabidopsis Col-0 plants at 10, 24, and 48 h following MgCl₂ treatment, Psm inoculation, or Psm avrRpm1 inoculation (OD₆₀₀ = 0.005 each) and in systemic leaves at 48 h post treatment. Details are as described for Figure 3. Amounts of (D)MPBQ in leaves are given relative to the mean of the 48-h mock samples, which consequently has a numerical value of 1. MPBQ and DMPBQ were identified according to their mass spectra (Supplemental Fig. S7). B and C, Relative amounts of MPBQ (top) and DMPBQ (bottom) in leaves of different mock-treated or Psm-inoculated Arabidopsis lines at 48 h post treatment. Different letters above the bars denote statistically significant differences of the values of each subfigure (P < 0.05, ANOVA and posthoc Tukey’s HSD test). Other details are as described in Figure 5. Amounts of (D)MPBQ in leaves are given relative to the mean of the Col-0 48-h Psm samples, which as a consequence has a numerical value of 1.