Figure 9.

vte2 mutants exhibit increased oxidation of triunsaturated lipids following Psm infection. A, The generation of TBA-reactive aldehydes was assessed in Arabidopsis Col-0 plants as a marker for lipid peroxidation in untreated leaves, in leaves infiltrated with 10 mm MgCl₂, and in leaves inoculated with Psm or Psm avrRpm1 (OD₆₀₀ = 0.005 each) in both Col-0 and vte2-2 plants at 48 h post treatment. Values represent means ± sd of three replicate leaf samples from different plants. Each replicate sample consisted of six leaves (three leaves from two plants). Peroxidation levels were normalized to the level measured in untreated Col-0 leaves. Different letters above the bars denote statistically significant differences (P < 0.01, ANOVA and posthoc Tukey’s HSD test). B, Assessment of lipid peroxidation in leaves of mock-treated or Psm-inoculated Col-0, vte2-1, vte2-2, and fad3-2 fad7-2 fad8 plants as well as in two different lines (59/60 and 63/64) of the quadruple mutant fad3-2 fad7-2 fad8 vte2-1 at 48 h post treatment. Details are as described in A.