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. 2019 Sep 18;181(3):945–960. doi: 10.1104/pp.19.00629

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Figure 4. — Substrate plasticity of AMO and GAOs against seven sesquiterpene substrates synthesized by six sesquiterpene synthases. Top shows immunoblot analysis for the expression of AMO (A), BsGAO (B), LsGAO1 (L1), and LsGAO2 (L2) in EPY300 coexpressing these P450s, CPR, and each of the seven sesquiterpene synthases. A, (−)-LC-MS metabolite profiling of the transgenic EPY300 yeasts. The coexpressed sesquiterpene synthases and P450s are indicated on top and at the far right, respectively. The extracted ions are m/z 171 for decanoic acid (asterisk, internal standard), m/z 233 for sesquiterpene acids (diamond), and m/z 251 for hydrated sesquiterpene acids (triangle). B, (+)-LC-MS metabolite profiles from transgenic EPY300 yeasts.