Figure 7.
LC-MS analyses of the sesquiterpenoids from transgenic yeasts cultivated in buffered and unbuffered media. A, Yeast strains expressing LsGAO2/CPR together with one of the six sesquiterpene synthases were cultivated in unbuffered medium and HEPES-buffered medium, and sesquiterpenoid metabolites were detected by (−)-LC-MS. Triangles indicate compounds with m/z (−)-251, [M+H2O-H]− ion, and diamonds indicate compounds with m/z (−)-233, [M-H]− ion. B, Disappearance of the compound with m/z (+)-239 (rectangle) under buffered medium is shown.