Figure 8.

Assessing catalytic activities of native and mutant GAOs. A, Relative catalytic activities to convert germacrene A and amorphadiene to respective sesquiterpene acids by native and mutant forms of BsGAO and LsGAO1. Catalytic activities of the native enzymes were set as 1, which are defined as GAS/BsGAO = 27 µg mL⁻¹, GAS/LsGAO1 = 22 µg mL⁻¹, ADS/BsGAO = 20 µg mL⁻¹, ADS/LsGAO1 = 27 µg mL⁻¹. Data are means ± sd (n = 3–4; biological replicates). B, Immunoblot analyses of native and mutant forms of BsGAO and LsGAO1. FLAG-epitope was tagged to the C terminus of BsGAO and LsGAO1, and monoclonal antibodies were used to detect the recombinant enzymes.