Figure 1.

Cryo-EM reconstruction of synthetic shells. A, Example cryo-EM micrograph used in reconstruction with example particles of different shell types boxed (T = 4 in magenta, T = 3 in yellow, and T = 4, Q = 6 prolate in cyan). All particles were used for reconstruction, but only selected ones are highlighted for clarity. B, Single-particle analysis preprocessing workflow with example 2D class averages for the different shell types. Particle counts and percentage of input particles for each class are indicated. C to E, 3D reconstructions for the T = 4 (C), T = 3 (D), and prolate (E) shell types with dimensions indicated.