Figure 2.

Assay for TcPYS activity of the candidate cytochrome P450 oxidoreductases identified in our coexpression analysis. The assays were performed by transiently expressing the candidate genes in N. benthamiana for 3 d via agrobacterium infiltration and immersing their leaves in 2 mm of jasmone for 24 h, after which the leaves were ground and extracted with MTBE. Detection of jasmolone and pyrethrolone in the MTBE extracts were by GC-MS (total ion mode). The jasmolone and pyrethrolone standards were obtained as described in Li et al. (2018). Pyrethrolone was detected only in leaves coexpressing candidate gene DN144246 with TcJMH.