Coexpression of TcJMH and TcPYS in Arabidopsis. A, GC-MS detection of jasmolone and pyrethrolone in a line stably expressing TcJMH (JMH), two lines expressing TcPYS (line PYS1 and PYS2), and two lines expressing both genes (JMH × PYS1 and JMH × PYS2). Wild-type Col-0 line was used as control. TIC, total ion chromatogram; SIM, single ion monitoring. B, RT-qPCR analysis of relative TcJMH and TcPYS transcript levels in lines analyzed in (A). C, Quantification of jasmolone and pyrethrolone levels in the respective lines; N.D., not detected. Data are presented as means ± sd (n = 3).