Figure 5.

In vitro activity and substrate specificity assays for TcPYS. In vitro conversion of jasmolone to pyrethrolone in microsomes prepared from N. benthamiana leaves transiently expressing TcPYS. Substrate specificity was tested on chemicals with similar structures including jasmone, MeJA, hydroxy-4-methyl-7-cis-decenoic acid γ-lactone, cis-3-Hexenyl 3-methylbutanoate, cis-3-hexenyl benzoate, and dihydrojasmone. The reaction was carried out overnight, after which the solution was extracted with MTBE and the extract analyzed by GC-MS.