AtTMS is recruited to the PM by SYP124/125/131/132. A, AtTMS displayed a sharp PM localization when coexpressed with mCherry-SYP124FL (first row) and a largely cytosolic pattern with mCherry-SYP124ΔTM (second row). At far right for each row, the fluorescence intensity along the white lines is measured by a plot profile plugin for ImageJ. PM localization is indicated by arrows. B to E, AtTMS could be recruited by SYP125 (B), SYP131 (C), and SYP132 (D) but not by SYP112 (E). Bars = 5 µm.