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. 2019 Sep 17;78(11):1559–1565. doi: 10.1136/annrheumdis-2019-215210

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© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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TNFα and IL-17A are produced by both γδT-cell subsets quantitative PCR showing TNFα and IL-17A transcript expression in γδT-cell subsets with or without stimulation (n=8) (A). Fold induction of secreted IL-17A protein following PMA/ionomycin stimulation compared with unstimulated fraction of purified Vδ1 and Vδ2 subsets (n=3) bar shows that mean whiskers represent 1 SD (B). Transcript expression of IL-17A, IL-17F and IL-22 following 48-hour stimulation of Vδ1 and Vδ2 subsets with combined anti-CD3/CD28 or IL-23 stimulation (n=10). Line denotes mean expression (all genes combined) (C). Genes for which a housekeeping value was obtained, but for which a target value was not, were assigned a value of 0.0001, as 0 cannot be plotted on a log scale. *P<0.05, **P<0.01, ***P≤0.001.