Fig 3. Neutralization of CHIKV by human mAbs.
(A) Volcano plot of 46 human mAbs for their ability to inhibit infection of CHIKV 181/25 at 30 and 300 nM. (B) IC50 values for 19 of the mAbs against CHIKV 181/25. The error bars represent 95% confidence interval from data fitting, all IC50 values were measured twice independently with similar results. (C) Neutralization of ESCA genotype LR2006_OPY1 and Asian genotype AF15561 by human mAbs. (D) Fusion assay in which CHKV 181/25 was first bound to cells, the mAb added, and the pH lowered to pH 5.5 for 2 min at 37 oC to trigger virus fusion at the plasma membrane. (E) Post-attachment assay, which was similar to (D), but following mAb addition, the virus was allowed to enter cells by endocytosis. (F) Cross-neutralization of MAYV by human CHIKV mAbs. A representative dataset from two experiments is shown; points represent mean ± SD.