Figure 2.

Photomicrographs showing neuro2a cells (A). In order to determine the condition of neurites, phase-contrast photomicrographs of the cells were taken. The scale bar represents 10 µm. AAPH induces death of neuro2a cells (B). Neuro2a cells were treated with various concentrations of AAPH. After 24 hours, the number of dead neuro2a cells was counted using the trypan blue dye exclusion assay. Each point represents the mean of three independent experiments. At least three wells were counted per experiment. Data were analyzed using the Student's t-test (*P < 0.05, **P < 0.01) and one-way ANOVA.