Figure 3.

Treatment of neuro2a cells with low concentrations of AAPH induces neurite degeneration. Neuro2a cells were treated with various concentrations of AAPH. After 24 hours, the cells were fixed with 4% paraformaldehyde (PFA) in PBS. Photomicrographs of the cells were collected and analyzed on a personal computer. The scale bar represents 10 µm. Arrows indicate bead formation on the degenerating neurites of neuro2a cells (A). The results of quantitative analysis of neurite degeneration are shown (B). Each column represents the mean of three independent experiments. At least three wells were examined per experiment. Data were analyzed using the Student's t-test (⁺P < 0.05 between normal neurites at 0 and 1 mM AAPH, and 0 and 2 mM AAPH, respectively. *P < 0.05, **P < 0.01 between altered neurites at 0 and 0.5 mM AAPH, 0 and 0.7 mM AAPH, 0 and 1 mM AAPH, and 0 and 2 mM AAPH. ^#P < 0.05 between the total number of neurites in neurons treated with 0 and 2 mM AAPH) and one-way ANOVA.