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C457S of Pg is potentially redox-sensitive. (a, b) MC38 cells transfected with mock vector or either WT, C420S, or C457S Pg were treated with 1 mM H2O2 and the Pg protein level was evaluated. n = 2 for each group. (c, d) Proteins extracted from MC38 cells transfected with mock vector or WT, C420S, or C457S Pg were treated with 0 or 1 mM H2O2 and the oxidation of Pg was detected and analyzed using the BIAM-SA method. Values are presented as mean ± SEM.
