C457S alters the cellular localization of Pg. (a–c) Cellular distributions of E-cadherin (green), Pg (red), α-catenin (red), and β-catenin (red) were examined in MC38 cells expressing WT, C420S, and C457S Pg using immunofluorescent analysis. DAPI (blue) was used to stain cell nuclei. Scale bar = 10 μm.