Table 1. ROS production, lipid peroxidation, protein carbonylation, and antioxidant parameters in the absence (NaAsO2) and presence of AEEF (AEEF + NaAsO2) in isolated mouse hepatocytes.
| Parameters | ||||||||
|---|---|---|---|---|---|---|---|---|
| Groups | TBARS (µg/g of protein) | Protein carbonyl (nmol/mg of protein) | CAT (U/mg of protein) | SOD (U/mg of protein) | GST (µmol/h/mg protein) | GR (nmol/min/mg of protein) | G6PD (nmol/min/mg of protein) | GSH (nmol/mg of protein) |
| Control | 2.36 ± 0.25 | 38.53 ± 2.65 | 197.03 ± 16.48 | 74.45 ± 2.25 | 1.36 ± 0.11 | 75.78 ± 3.46 | 106.42 ± 4.26 | 5.82 ± 0.51 |
| NaAsO2 (10 µM) | 5.31 ± 0.32* | 71.08 ± 2.05* | 109.19 ± 12.92* | 55.53 ± 2.68* | 0.91 ± 0.07* | 37.66 ± 4.85* | 70.83 ± 6.00* | 3.29 ± 0.38* |
| NaAsO2 (10 µM) + AEEF (400 µg/ml) | 3.64 ± 0.29‡ | 45.46 ± 2.54‡ | 178.24 ± 16.01‡ | 68.77 ± 3.30‡ | 1.23 ± 0.09† | 62.54 ± 3.04‡ | 92.91 ± 3.73‡ | 5.05 ± 0.56† |
Values are expressed as mean ± SE (n = 3).
*Values differ significantly from normal control (P < 0.01).
†Values differ significantly from NaAsO2 control (P < 0.05).
‡Values differ significantly from NaAsO2 control (P < 0.01).
CAT unit, ‘U’, is defined as micromoles of H2O2 consumed per minute. SOD unit, ‘U’, is defined as the micromoles inhibition of nitroblue tetrazolium reduction per minute.