Table 3. ROS production, lipid peroxidation, protein carbonylation, and antioxidant markers in the absence (NaAsO2) and presence of AEEF (AEEF + NaAsO2) in mouse liver.
| Groups | Parameters | Values | Parameters | Values |
|---|---|---|---|---|
| Normal control | ROS production (nmol min/mg of protein) | 31.42 ± 1.48 | CAT (U/mg of protein) | 264.00 ± 18.05 |
| Toxic control (NaAsO2) | 88.43 ± 2.97* | 177.83 ± 24.10* | ||
| NaAsO2 (5 mg/kg) + AEEF (50 mg/kg) | 50.45 ± 2.83‡ | 234.67 ± 13.51 | ||
| NaAsO2 (5 mg/kg) + AEEF (100 mg/kg) | 43.57 ± 2.64‡ | 246.83 ± 12.55† | ||
| Normal control | Lipid peroxidation (TBARS level in μg/g of protein) | 4.76 ± 0.40 | SOD (U/mg of protein) | 66.25 ± 1.79 |
| Toxic control (NaAsO2) | 8.92 ± 0.67* | 35.87 ± 2.64* | ||
| NaAsO2 (5 mg/kg) + AEEF (50 mg/kg) | 7.21 ± 0.62 | 54.35 ± 4.25‡ | ||
| NaAsO2 (5 mg/kg) + AEEF (100 mg/kg) | 5.85 ± 0.37‡ | 58.67 ± 3.95‡ | ||
| Normal control | Protein carbonylation (nmol/mg of protein) | 33.56 ± 2.89 | GST (µmol/h/mg protein) | 2.25 ± 0.16 |
| Toxic control (NaAsO2) | 60.31 ± 4.38* | 1.37 ± 0.14* | ||
| NaAsO2 (5 mg/kg) + AEEF (50 mg/kg) | 40.09 ± 3.09‡ | 1.81 ± 0.13 | ||
| NaAsO2 (5 mg/kg) + AEEF (100 mg/kg) | 37.11 ± 4.68‡ | 2.03 ± 0.11‡ | ||
| Normal control | Total coenzyme Q9 (nmol/g of wet tissue) | 184.31 ± 7.31 | GR (nmol/min/mg protein) | 82.60 ± 4.78 |
| Toxic control (NaAsO2) | 133.85 ± 5.45* | 47.71 ± 6.68* | ||
| NaAsO2 (5 mg/kg) + AEEF (50 mg/kg) | 155.63 ± 4.67† | 73.89 ± 6.09† | ||
| NaAsO2 (5 mg/kg) + AEEF (100 mg/kg) | 161.32 ± 4.89‡ | 76.97 ± 9.86† | ||
| Normal control | Total coenzyme Q10 (nmol/g of wet tissue) | 33.42 ± 2.55 | G6PD (nmol/min/mg protein) | 92.88 ± 3.62 |
| Toxic control (NaAsO2) | 19.46 ± 1.98* | 64.08 ± 4.57* | ||
| NaAsO2 (5 mg/kg) + AEEF (50 mg/kg) | 25.79 ± 2.67 | 78.46 ± 3.02† | ||
| NaAsO2 (5 mg/kg) + AEEF (100 mg/kg) | 28.43 ± 2.32‡ | 81.12 ± 4.52† | ||
| Normal control | NADPH oxidase (pmol/min/mg of protein) | 0.42 ± 0.03 | GSH (nmol/mg protein) | 20.48 ± 1.88 |
| Toxic control (NaAsO2) | 3.42 ± 0.52* | 10.50 ± 0.82* | ||
| NaAsO2 (5 mg/kg) + AEEF (50 mg/kg) | 2.41 ± 0.28† | 15.44 ± 1.11† | ||
| NaAsO2 (5 mg/kg) + AEEF (100 mg/kg) | 2.48 ± 0.25† | 17.64 ± 1.42‡ |
Values are expressed as mean ± SE, for six animals in each group. CAT unit, ‘U’, is defined as micromoles of H2O2 consumed per minute. SOD unit, ‘U’, is defined as the micromoles inhibition of NBT reduction per minute.
*Values differ significantly from normal control (P < 0.01).
†Values differ significantly from NaAsO2 control (P < 0.05).
‡Values differ significantly from NaAsO2 control (P < 0.01).