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Aqueous extract of P. hexandrum induced DNA fragmentation in HL-60 cells. Cells 2 × 106/ml/well were treated with indicated conc. of plant extract for 24 hours. Genomic DNA was isolated and put to electrophoresis as described in the Materials and methods to assess the fragmentation at different concentrations. Lane 1, 1 kb ladder; Lane 2, –ve control (untreated cells); Lane 3, +ve control (camptothecin at 5 µM for 6 hours); Lanes 4–7, extract at 100, 70, 50, 30 µg/ml.
