Figure 3.

Coagulation times, lipid oxidation (MDA), and ceruloplasmin curve. (A) Coagulation times of different treated groups. PTT and PT were analyzed. There were no significant differences between fibrotic and EDTA-treated animals. (B) Lipid peroxidation measurement by the MDA technique. Significant decrease in lipid peroxidation was observed in fibrotic animals treated with EDTA (60 mg/kg) in the preventive and therapeutic EDTA groups with respect to fibrotic control. (C) Activity of Cp at 2, 6, and 11 weeks of CCl₄ treatment. Cp activity diminished gradually in serum concomitantly with time of CCl₄ insult. (D) Activity of Cp at 2 weeks of CCl₄ intoxication with or without concomitant administration of EDTA (60 mg/kg). EDTA treatment groups maintained Cp activity even in the presence of a continued administration of CCl₄. (E) Activity of Cp at 6 weeks of CCl₄ intoxication with or without concomitant administration of EDTA (60 mg/kg). As the time of intoxication advanced, Cp activity increased in the EDTA-treated group (62%). (F) Cp activity in therapeutic and preventive groups. EDTA treatment is associated with increased Cp activity. The Cp activity in the normal EDTA group was not statistically different with respect to the normal group in all assays (data not shown). Cp activity was determined by zymography assay. Activities in the gel slabs were quantified (relative units area) using an image analyzer system (Kodak 1D 3.5 image analyzer). Values are the mean ± standard deviation of the mean of five rats per group. Asterisks indicate values significantly different (*P ≤ 0.05).