Fig 5. PfUCH37 D38E mutants and wild type parasites respond similarly to drug challenge.

PfUCH37 D38E mutants, D38D controls and the Dd2 wild type parent line were treated for 72-h with a 2-fold serial dilution of A) dihydroartemisinin (from 50 nM to 0.1 nM), B) chloroquine (10 μM to 0.02 μM), C) methylene blue (100 nM to 0.2 nM) or D) MLN4924 (200 μM to 0.391 μM) and parasite growth measured by SYBR green assay. In panel D, the dotted line corresponds to the equivalent concentration of DMSO, which at the highest concentration used (1% v/v) has an impact on parasite growth. Data reported as means ± standard errors with n≥ 3.