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. 2019 Oct 28;17(10):e3000379. doi: 10.1371/journal.pbio.3000379

Fig 4. CmrT is required for rough colony formation.

Fig 4

(A) Colony morphology of C. difficile R20291, ΔcmrR, and ΔcmrT with vector after 24 hours on BHIS-1.8% agar. (B) Complementation of ΔcmrR and ΔcmrT mutants with expression of the respective genes in trans induced with 2 ng/mL ATc, imaged after 24 hours. All images were taken at ×2 magnification. Representative images from at least two independent experiments are shown. ATc, anhydrotetracycline; BHIS, brain heart infusion plus yeast; cmr, colony morphology regulators.