Figure 3.
A) Venn diagram of the 92 AADMtotal subjects identified by each of the AADMs. At least 50% of the subjects identified by one AADM were identified by the other 2 AADMs. The AADMtotal population is comprised of 86 ASD and 6 TYP subjects. The overall prevalence of metabolic dysregulation in the CAMP ASD population is 16.7% (86 AADMtotal ASD / 516 CAMP ASD), specificity 96.3 % (158 AADM-negative TYP / 164 CAMP TYP), PPV 93.5% (86 AADMtotal ASD / 92 AADMtotal). B) PCA analysis of the metabolite ratios used in the metabolic signature of the reproducible AADMs creating the AADMtotal estimates in the CAMP study population. Black circle is the 95% confidence interval from the Hoetellings T2. Red letters are AADMtotal positive (N=92), Black letters are AADMtotal negative (N=588). A=ASD and T=TYP. Abbreviations: BCAA, branched chain amino acid; Orn, Ornithine; ASD, autism spectrum disorder; TYP, typically developing; AADM, amino acid dysregulation metabotype; CAMP, Children’s Autism Metabolome Project.