Table 1:
Diagnostic performance metrics of amine ratios to discriminate subpopulations of ASD subjects in the training and test sets of subjects. The ratios all include branched chain amino acid (BCAA) values in the denominators and negatively correlated Gly-cluster metabolites in the numerator. Abbreviations: Pos., positive, Pred., predictive; AADM, amino acid dysregulation metabotype; ASD, autism spectrum disorder; Train, training set; Test, test set.
Ratio | Sensitivity | Specificity | Pos. Pred. Value | Permutation Test | ||||
---|---|---|---|---|---|---|---|---|
Train | Test | Train | Test | Train | Test | Train | Test | |
Ratios used to create AADMAlanine | ||||||||
Ala:Ile | 0.202 | 0.175 | 0.894 | 0.937 | 0.850 | 0.902 | 3.40E-02 | 9.00E-03 |
Ala:Leu | 0.245 | 0.133 | 0.894 | 0.937 | 0.873 | 0.875 | 2.00E-03 | 5.50E-02 |
Ala:Val | 0.178 | 0.114 | 0.918 | 0.924 | 0.865 | 0.833 | 2.20E-02 | 2.22E-01 |
Ratios used to create AADMGlutamine | ||||||||
Gln:Ilea | 0.126 | 0.163 | 0.976 | 0.937 | 0.941 | 0.896 | 3.00E-03 | 1.30E-02 |
Gln:Leu | 0.103 | 0.217 | 0.976 | 0.886 | 0.929 | 0.864 | 9.00E-03 | 2.40E-02 |
Gln:Val | 0.130 | 0.186 | 0.976 | 0.886 | 0.943 | 0.845 | 1.00E-03 | 9.90E-02 |
Ratios used to create AADMGlycine | ||||||||
Gly:Ilea | 0.174 | 0.152 | 0.953 | 0.962 | 0.917 | 0.930 | 0.00E+00 | 2.00E-03 |
Gly:Leua | 0.146 | 0.148 | 0.953 | 0.987 | 0.902 | 0.975 | 8.00E-03 | 0.00E+00 |
Gly:Val | 0.126 | 0.160 | 0.976 | 0.937 | 0.941 | 0.894 | 3.00E-03 | 1.50E-02 |
Ratios used to create AADMHomoserine | ||||||||
Hse:Ile | 0.063 | 0.137 | 0.976 | 0.949 | 0.889 | 0.900 | 1.16E-01 | 2.50E-02 |
Hse:Leu | 0.107 | 0.122 | 0.953 | 0.975 | 0.871 | 0.941 | 8.20E-02 | 3.00E-03 |
Hse:Val | 0.067 | 0.160 | 0.965 | 0.962 | 0.850 | 0.933 | 2.09E-01 | 4.00E-03 |
Ratios used to create AADMOrnithine | ||||||||
Orn:Ilea | 0.115 | 0.103 | 0.965 | 0.987 | 0.906 | 0.964 | 2.10E-02 | 9.00E-03 |
Orn:Leua | 0.103 | 0.202 | 0.965 | 0.949 | 0.897 | 0.930 | 3.70E-02 | 0.00E+00 |
Orn:Val | 0.119 | 0.141 | 0.953 | 0.937 | 0.882 | 0.881 | 3.70E-02 | 4.30E-02 |
Ratios used to create AADMSerine | ||||||||
Ser:Ile | 0.130 | 0.129 | 0.953 | 0.949 | 0.892 | 0.895 | 2.00E-02 | 2.20E-02 |
Ser:Leua | 0.138 | 0.167 | 0.941 | 0.924 | 0.875 | 0.880 | 3.00E-02 | 3.80E-02 |
Ser:Val | 0.190 | 0.228 | 0.941 | 0.873 | 0.906 | 0.857 | 1.00E-03 | 3.90E-02 |
Ratios used to create AADMHydroxyproline | ||||||||
Hyp:Ile | 0.111 | 0.087 | 0.941 | 0.911 | 0.848 | 0.767 | 1.14E-01 | 6.03E-01 |
Hyp:Leu | 0.115 | 0.095 | 0.918 | 0.899 | 0.806 | 0.758 | 2.69E-01 | 6.64E-01 |
Hyp:Val | 0.241 | 0.213 | 0.871 | 0.772 | 0.847 | 0.757 | 1.70E-02 | 6.64E-01 |
The observed diagnostic performance occurred in less than 5% of 1000 permutations of subject diagnosis in both training and test sets.