Table 1.

Mutation rates caused by Exo1 MIP and SHIP mutations at the Exo1 genomic locus

RDKY number	Relevant Genotype	Thr+	Lys+	CanR
RDKY5964	Wild-type*	2.26 [1.22–3.30] × 10−9 (1)	1.01 [0.67–1.86] × 10−8 (1)	5.65 [3.56–8.53] × 10−8 (1)
RDKY3688	msh2Δ*	3.24 [2.60–4.33] × 10−6 (1,433)	9.38 [7.30–14.0] × 10−5 (9,287)	2.98 [2.43–4.38] × 10−6 (53)
RDKY7884	exo1Δ*	4.93 [3.04–10.2] × 10−9 (2)	4.02 [2.67–7.34] × 10−8 (4)	2.58 [1.52–11.2] × 10−7 (5)
RDKY8075	pol30-K217E*	9.27 [7.47–18.0] × 10−9 (4)	3.26 [2.39–5.33] × 10−7 (32)	3.99 [3.43–5.98] × 10−7 (7)
RDKY8077	pol30-K217E exo1Δ*	1.66 [1.39–3.54] × 10−6 (735)	4.04 [2.80–5.94] × 10−5 (4,000)	4.76 [3.53–7.48] × 10−6 (84)
RDKY9358	pol30-K217E exo1-MIP	5.78 [4.56–10.9] × 10−8 (26)	2.79 [2.16–5.18] × 10−6 (275)	8.14 [6.64–11.4] × 10−7 (14)
RDKY9359	pol30-K217E exo1-SHIP1	1.72 [1.42–2.43] × 10−8 (8)	4.54 [3.48–5.81] × 10−7 (45)	5.94 [4.74–10.7] × 10−7 (11)
RDKY9360	pol30-K217E exo1-SHIP2	2.08 [1.40–3.66] × 10−8 (9)	5.78 [3.24–13.2] × 10−7 (57)	6.59 [3.51–10.4] × 10−7 (12)
RDKY9361	pol30-K217E exo1-SHIP1, SHIP2	5.06 [2.66–22.1] × 10−8 (22)	1.37 [0.84–16.1] × 10−6 (135)	1.18 [0.56–2.47] × 10−6 (21)
RDKY9362	pol30-K217E exo1-MIP, SHIP1	3.23 [2.40–4.94] × 10−7 (143)	7.89 [5.68–10.2] × 10−6 (778)	2.15 [1.12–5.08] × 10−6 (38)
RDKY9363	pol30-K217E exo1-MIP, SHIP2	5.59 [2.31–9.97] × 10−7 (247)	1.21 [0.53–2.12] × 10−5 (1195)	1.60 [0.79–2.52] × 10−6 (28)
RDKY9364	pol30-K217E exo1-MIP, SHIP1, SHIP2	1.33 [1.12–1.88] × 10−6 (589)	4.10 [3.32–5.40] × 10−5 (4,043)	2.88 [2.46–4.68] × 10−6 (51)

Reported rates are the median rates with 95% confidence interval in square brackets. Fold increase in mutation rate over the wild-type strain rate (RDKY5964) is listed in parenthesis. Total loss of mismatch repair is represented by msh2Δ (RKDY3688). n=14 independent cultures from two independently derived isolates.

^*measured in Goellner et. al. Molecular Cell 2011