Table 2.
Mutation rates caused by dpb3Δ, fun30Δ and rsc2Δ mutations
| RDKY number |
Relevant Genotype | Thr+ | Lys+ |
|---|---|---|---|
| RDKY5964 | Wild-type* | 2.26 [1.22–3.30] × 10−9 (1) | 1.01 [0.67–1.86] × 10−8 (1) |
| RDKY9371 | dpb3Δ | 4.74 [1.33–189] × 10−9 (2.1) | 1.33 [0.54–2.39] × 10−8 (1.3) |
| RDKY9367 | fun30Δ | 5.3 [0.91–8.71] × 10−9 (2.3) | 2.84 [1.72–5.01] × 10−8 (2.8) |
| RDKY9409 | rsc2Δ | 3.8 [2.61–7.20] × 10−9 (1.7) | 2.74 [0.77–3.47] × 10−8 (2.7) |
| RDKY9410 | fun30Δ rsc2Δ | 9.83 [4.25–64.3] × 10−9 (4.3) | 5.27 [3.23–14.0] × 10−8 (5.2) |
| RDKY7884 | exo1Δ* | 4.93 [3.04–10.2] × 10−9 (2.2) | 4.02 [2.67–7.34] × 10−8 (4) |
| RDKY9372 | exo1Δ dpb3Δ | 7.53 [3.09–12.5] × 10−9 (1.5) | 5.14 [4.74–6.36] × 10−8 (5) |
| RDKY9368 | exo1Δ fun30Δ | 1.06 [0.62–9.38] × 10−8 (4.7) | 7.28 [4.51–23.7] × 10−8 (7.2) |
| RDKY9411 | exo1Δ rsc2Δ | 3.38 [2.34–7.88] × 10−8 (15) | 1.05 [0.8–1.23] × 10−6 (100) |
| RDKY9412 | exo1Δ fun30Δ rsc2Δ | 1.96 [1.16–4.00] × 10−8 (8.7) | 2.79 [1.78–4.08] × 10−7 (28) |
| RDKY8075 | pol30-K217E* | 9.27 [7.47–18.0] × 10−9 (4) | 3.26 [2.39–5.33] × 10−7 (32) |
| RDKY9373 | pol30-K217E dpb3Δ | 1.54 [0.98–2.31] × 10−8 (6.8) | 4.89 [3.75– 9.84] × 10−7 (48) |
| RDKY9369 | pol30-K217E fun30Δ | 4.39 [3.02–6.01] × 10−8 (19) | 8.34 [6.49–10.7] × 10−7 (83) |
| RDKY9413 | pol30-K217E rsc2Δ | 7.46 [3.21–25.9] × 10−8 (33) | 3.66 [2.85–6.63] × 10−6 (362) |
| RDKY9414 | pol30-K217E fun30Δrsc2Δ | 1.36 [0.97–6.18] × 10−7 (60) | 3.38 [1.54–4.10] × 10−6 (335) |
Reported rates are the median rates with 95% confidence interval in square brackets. Fold increase in mutation rate is listed in parenthesis as compared to the wild-type strain. ). n=14 independent cultures from two independently derived isolates.
*
measured in Goellner et. al. Molecular Cell 2014