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. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: Nat Methods. 2019 Jun 17;16(7):619–626. doi: 10.1038/s41592-019-0433-8
Dataset PCs Variable gene dispersion threshold Variable gene expression threshold
Proof-of-concept, live cells 8 0.5 0.025
Proof-of-concept, human nuclei 8 0.5 0.0125
Proof-of-concept, mouse nuclei 6 0.5 0.0125
Proof-of-concept, Jurkat nuclei 10 0.5 0.0125
96-Plex HMEC, all cells 8 0.65 0.05
96-Plex HMEC, all LEPs 9 0.4 0.05
96-Plex HMEC, all MEPs 10 0.75 0.05
96-Plex HMEC, resting LEPs 8 0.4 0.05
96-Plex HMEC, resting MEPs 6 1.0 0.05
PDX, mouse immune cells 15 0.75 0.05
PDX, mouse lung immune cells 14 0.7 0.05
PDX, classical monocytes 10 0.53 0.05
PDX, late-stage classical monocytes 6 0.65 0.05