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. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: Cell Rep. 2019 Jul 2;28(1):190–201.e3. doi: 10.1016/j.celrep.2019.06.003

Figure 3. Cell Proliferation in the Injured Hearts.

Figure 3.

(A) Diagram of pulse-chase EdU labeling of proliferating cells and the timeline for EdU injection and tissue collection.

(B–I) Co-localization of EdU, cTNT, and nuclei (DAPI) in the apex (B1, D1, F1, and H1) and remote areas (B2, D2, F2, and H2) at 21–32 dps (B–E) and 32–43 dps

(G–I) in the resected (B, C, F, and G) and sham (D, E, H, and I) groups. Asterisks indicate injury site. Arrows in (C1) and (G1) show EdU-positive cardiomyocytes, and arrows in (C2), (E), (G2), and (I) show EdU-positive non-cardiomyocytes (cTnT−).

(J and K) Quantification of EdU-labeled proliferative cells (J) and cardiomyocytes (K) in sham and resected hearts at 32 dps and 43 dps.

Quantitative analysis was performed on 5 fields at 100× magnification from 3 different hearts per group at each time point. *p<0.05, **p < 0.01 versus the control.

Scale bar, 100 μm (white) and 25 μm (yellow).

See also Figure S3.