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. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: Cell Rep. 2019 Jul 2;28(1):190–201.e3. doi: 10.1016/j.celrep.2019.06.003

Figure 5. Tbx18+ Cells Do Not Become Cardiomyocytes or Coronary Endothelial Cells after Birth.

Figure 5.

The neonatal mice were given a single subcutaneous dose of tamoxifen at P0, and the hearts were collected at postnatal day 30 for analysis.

(A and B) Tbx18 progeny do not include cardiomyocytes (not co-localized with cTNT, A, or Nkx2.5, B).

(C and D) Co-expression of Tbx18 progeny with SM-MHC (C4) and α-SMA (D4).

(E) Tbx18 lineage is not co-expressed with PECAM in the coronary endothelial cells (E4).

(A1), (B1), (C1), (D1), and (E1) are DAPI staining. GFP-positive cells in (A2), (B2), (C2), (D2), and (E2) are cells of Tbx18 lineage. (A3), (B3), (C3), (D3), and (E3) are antibody staining for cTNT, Nkx2.5, SM-MHC, α-SMA and PECAM, respectively. (A4), (B4), (C4), (D4), and (E4) are overlays of (A1–A3), (B1–B3), (C1–C3), (D1–D3), and (E1–E3), respectively. Scale bar, 100 μm.

See also Figure S6.