KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken anti-GFP | Abcam | Cat# ab13970; RRID:AB_300798 |
| Mouse monoclonal anti-cTnT | DSHB | Cat#TI-1; RRID:AB_2206413 |
| Rat anti-CD31 | BD PharMingen | Cat# 550274; RRID: AB_393571 |
| Rabbit monoclonal anti-PDGFRβ | Abcam | Cat#ab32570; RRID:AB_777165 |
| Mouse monoclonal anti-α-SMA | Sigma-Aldrich | Cat#A5228; RRID:AB_262054 |
| Goat anti-Nkx2.5 | Santa Cruz Biotechnology | Cat#SC-8697; RRID:AB_650280 |
| Rabbit anti-SM-MHC | Biomedical Technologies Inc | Cat#BT-562; RRID:AB_10013421 |
| Rabbit polyclonal anti-Tbx18 | Abcam | Cat#ab115262 |
| Mouse monoclonal anti-CD90 | Abcam | Cat#ab225; RRID:AB_2203300 |
| Mouse monoclonal anti-PDGFRα | Abcam | Cat#ab96569; RRID:AB_10687154 |
| WGA-Alexa488 | Invitrogen | Cat#W6748 |
| Alexa594 donkey anti-rabbit IgG | Thermo Fisher | Cat#A21207; RRID:AB_141637 |
| Alexa 488 goat anti-chicken IgG | Thermo Fisher | Cat#A11039; RRID:AB_142924 |
| Alexa594 donkey anti-goat IgG | Thermo Fisher | Cat#A11058; RRID:AB_2534105 |
| Alexa594 donkey anti-rat IgG | Thermo Fisher | Cat#A21209; RRID:AB_2535795 |
| Alexa 594 goat anti-mouse IgG2a | Thermo Fisher | Cat#A21135; RRID:AB_1500827 |
| Dylight 488 rabbit anti-chicken IgY | Abcam | Cat#Ab96955; RRID:AB_10679801 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat#T5648 |
| Bouin’s Solution | Sigma-Aldrich | Cat#HT10-1-32 |
| Weigert’s Iron Hematoxylin Set | Sigma-Aldrich | Cat#HT10-79 |
| 5-Bromo-4-chloro-3-indolyl-α-D-galactopyranoside | Sigma-Aldrich | Cat#16555 |
| Critical Commercial Assays | ||
| Click-iT EdU Cell Proliferation Assay Kit | Thermo Fisher | Cat#C35002 |
| Click-iT Plus TUNEL Assay for In Situ Apoptosis Detection | Thermo Fisher | Cat#C10617 |
| Masson’s trichrome staining | Sigma-Aldrich | Cat#HT15-1KT |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.129S4-Gt(ROSA)26Sortm1Sor /J | The Jackson Laboratory | JAX: 003474 |
| Mouse: B6;129-Gt(ROSA)26Sortm9(CAG-GFP*)Nat/J | The Jackson Laboratory | JAX: 026005 |
| Mouse: B6;129-Gt(ROSA)26Sortm7(CAG-tdTomato*)Nat/J | The Jackson Laboratory | JAX: 026006 |
| Mouse: B6.129(Cg)-Gt(ROSA)26 Sortm4(ACTB-tdTomato,-EGFP)Luo/J | The Jackson Laboratory | JAX: 007676 |
| Mouse: Tbx18H2B-GFP | Cai et al., 2008 | N/A |
| Mouse: Tbx18MerCreMer/+ | This paper | N/A |
| Mouse: SM-MHCCreERT2 | Wirth et al., 2008 | N/A |
| Mouse: Nfatc1Cre | Wu et al., 2012 | N/A |
| Software and Algorithms | ||
| Photoshop CS software | Adobe | https://www.adobe.com/cn/products/cs6/photoshop.html |