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. 2019 Oct 18;8:e49065. doi: 10.7554/eLife.49065

Figure 5. Tc-foxQ2 positive cells project through the central brain primordium and contribute to the central complex.

Immunohistochemistry visualizes EGFP (green) derived from the foxQ2-5’-line. Synapsin visualizes adult brain morphology (magenta in E,F) while nuclei are visualized by DAPI (blue in A’-C’’). (A–A’’) At NS14, the anterior-lateral-foxQ2-cluster consists of one NPC and a small number of progeny (orange arrowheads). (B–B’’, D) At NS15, more cells are marked by EGFP (orange arrowheads) and their projections join a Tc-foxQ2 positive axon bundle (white arrows in B, (D). (C–C’’) EGFP marked projections contribute to the central body in L5 larval brain, which is visualized by its surrounding glia cells (white arrowhead in C’). (E–F) EGFP marked projections contribute to the upper unit of the central body in the adult brain (white arrowhead in E) visualized by synapsin and surrounding glia (white arrowhead in F). (E’–F’) Another fascicle projects across the midline directly posterior of the central body (open arrowhead).

Figure 5.

Figure 5—figure supplement 1. foxQ2-5’-line marks cells of the stomodeum and the lateral parts of the labrum.

Figure 5—figure supplement 1.

Immunohistochemistry visualizes the EGFP (green) expressed by the foxQ2-5’-line. The outline is visualized with DAPI staining (blue). The expression of Tc-foxQ2 marked by EGFP from NS6 to NS15 is shown. (A–C’) The stomodeum is located posteriorly in anterior median region and cells of it except for the dorsal roof are marked (white arrowheads). (D–E’) At NS14 and NS15, additional marked epidermal cells in the lateral parts of the labrum are observed (open arrowheads).
Figure 5—figure supplement 2. The foxQ2-5’-line marks cells lateral of the stomodeum.

Figure 5—figure supplement 2.

Immunohistochemistry visualizes the EGFP (green) derived from the foxQ2-5’-line. The morphology of the brain is visualized with DAPI staining (blue). (A–A’) At NS13, several cells lateral to the stomodeum are observed (open arrowhead). (B–B’) At NS14, this group of cells project into the posterior circumesophageal commissure (open arrowhead).