Figure 8. Loss of Tc-foxQ2 function in novel imaging lines confirms the midline phenotype.

(A–A’’) In WT Ten-a-green embryos, three groups of cells are marked by EGFP: An anterior group (white circle), a posterior-lateral group (open arrowhead) and a posterior-median group (dashed circle). The central brain primordium is marked with Ten-a positive fascicles projecting across the midline (white arrow in A). (B–B’’) In Tc-foxQ2 RNAi, the Ten-a positive projections and the number of the marked cells is reduced (n = 4). (C–C’) In WT Tc-rx-5’-up line, the anterior median group of cells marked by DsRed project into the central brain (white circle and white arrowhead). (D–D’) In Tc-foxQ2 RNAi, the cell number in the anterior median group is strongly reduced (n = 6; white circle) and the marked brain commissures are absent (white arrowhead). The peripheral cells are reduced in number as well (n = 6; compare open arrowheads in C,D).

Figure 8—figure supplement 1. Characterization of imaging lines.

Shown are antibody stainings (left column), signal from transgenic reporter lines (medial column) and overlay (right column). Upper panels: Co-localization of reporter protein with endogenous Ten-a protein in the Ten-a-line. Middle panel: anti Ten-a staining in Tribolium embryo marks the first midline crossing fascicle (left panel). Later, it marks several midline crossing fascicles of the central brain (right panel). Lower panels: Partial co-localization of reporter protein with endogenous protein in the rx-5’-up line.