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. Author manuscript; available in PMC: 2020 Mar 22.
Published in final edited form as: J Nat Prod. 2019 Mar 7;82(3):469–484. doi: 10.1021/acs.jnatprod.9b00176

Figure 2.

Figure 2.

Predicted versus actual antimicrobial activity of A. keiskei spiked extract and pools at 100 μg/mL. Predicted antimicrobial activity was calculated by quantifying compounds 1-4 (berberine, magnolol, cryptotanshinone, and α-mangostin) in each pool and using these values to calculate predicted contribution to activity (via dose-response curves). Actual activity values represent percent growth inhibition of Staphylococcus aureus (SA1199)36 normalized to the vehicle control (broth containing bacteria but no antimicrobial compound) turbidimetric OD600 values. Data presented represent results of triplicate analyses ± SEM. Positive control data are the same as described for Figure 1.