FIG. 5.

Graph of intensity ratios for intracellular calcium indicator, Fluo 3-AM under different cell stimuli. Calcium remains unlocalized in the presence of a large chemoattractant gradient and in the absence of a chemical and electrical stimulus. When a field is applied, the voltage magnitude influences the relative localization of intracellular calcium for cells during electrotaxis, with calcium increasingly preferentially localizing at the front of the cell with increasing voltage. Cell numbers are shown on each bar and error bars represent the standard error of the means. **P < 0.01, ***P < 0.001.