Figure 6.

Reduced CD115 expressions on splenic Ly6C⁺CD11b⁺ monocytes in mice infected with WT sporozoites during the development of ECM. (A) Representative contour plots showing gating of Ly6C vs. CD115 expression on CD45⁺Ly6G⁻CD11b⁺ myeloid cells from naïve mice (left), and mice infected with WT (center) and PRF (right) sporozoites 8 days after infection. An arrow indicates the disappearance of CD115⁺Ly6C⁺ monocytes (blue circles) in spleens of mice infected with WT sporozoites. CD115⁻Ly6C⁺ monocytes are gated in gray circles. (B) Quantification of splenic CD115⁺Ly6C⁺ monocytes. Shown are percentage and numbers of CD115⁺Ly6C⁺ monocytes in the spleen. (C) Quantification of splenic CD115⁻Ly6C⁺ cells. Shown are percentage and numbers of CD115⁻Ly6C⁺ myeloid cells in the spleen. The scatter dot plots in (B,C) represent mean values (±SD) from samples (n = 8–11) isolated 8 days after infection from two independent experiments. n.s, non-significant; ^*P < 0.05; ^**P < 0.01; ^***P < 0.001 (Mann-Whitney test).